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NEWS & KNOWLEDGEMENT

How to Choose a HPLC Column?

Many factors are affecting the column selection in HPLC, including: Here, we’ll explain in more details on how each factor affect your HPLC column selection. Types of HPLC Columns

What is the purpose of the guard column?

Guard columns are recommended to protect analytical/preparative columns from contamination from injected particles, worn pump seals/injector rotor seals, or debris from unfiltered

How to determine dead volume in HPLC?

Dead volume (dead volume, V0) – the space from the injector inlet to the detector flow cell that is not occupied by the stationary phase. It includes 4 parts: the volume of t

What is HPLC?

HPLC is an abbreviation for High Performance Liquid Chromatography. “Chromatography” is a technique for separation, “chromatogram” is the result of chromato

Working Principle of HPLC Columns!

HPLC columns contain a stationary phase (usually porous silica particles) bonded to a support material to provide a large surface area. The stationary phase provides the basis for

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